The response of high and low polyamine-producing cell lines to aluminum and calcium stress.
Identifieur interne : 003077 ( Main/Exploration ); précédent : 003076; suivant : 003078The response of high and low polyamine-producing cell lines to aluminum and calcium stress.
Auteurs : Sridev Mohapatra [États-Unis] ; Smita Cherry ; Rakesh Minocha ; Rajtilak Majumdar ; Palaniswamy Thangavel ; Stephanie Long ; Subhash C. MinochaSource :
- Plant physiology and biochemistry : PPB [ 1873-2690 ] ; 2010.
Descripteurs français
- KwdFr :
- Aluminium (métabolisme), Aluminium (pharmacologie), Animaux (MeSH), Calcium (métabolisme), Espèces réactives de l'oxygène (métabolisme), Expression des gènes (MeSH), Lignée cellulaire (MeSH), Mitochondries (effets des médicaments et des substances chimiques), Mitochondries (métabolisme), Ornithine decarboxylase (métabolisme), Populus (génétique), Populus (métabolisme), Putrescine (métabolisme), Souris (MeSH), Stress oxydatif (MeSH), Stress physiologique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- effets des médicaments et des substances chimiques : Mitochondries.
- génétique : Populus.
- métabolisme : Aluminium, Calcium, Espèces réactives de l'oxygène, Mitochondries, Ornithine decarboxylase, Populus, Putrescine.
- pharmacologie : Aluminium.
- Animaux, Expression des gènes, Lignée cellulaire, Souris, Stress oxydatif, Stress physiologique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Aluminum (metabolism), Aluminum (pharmacology), Animals (MeSH), Calcium (metabolism), Cell Line (MeSH), Gene Expression (MeSH), Mice (MeSH), Mitochondria (drug effects), Mitochondria (metabolism), Ornithine Decarboxylase (metabolism), Oxidative Stress (MeSH), Plants, Genetically Modified (MeSH), Populus (genetics), Populus (metabolism), Putrescine (metabolism), Reactive Oxygen Species (metabolism), Stress, Physiological (MeSH).
- MESH :
- chemical , metabolism : Aluminum, Calcium, Ornithine Decarboxylase, Putrescine, Reactive Oxygen Species.
- chemical , pharmacology : Aluminum.
- drug effects : Mitochondria.
- genetics : Populus.
- metabolism : Mitochondria, Populus.
- Animals, Cell Line, Gene Expression, Mice, Oxidative Stress, Plants, Genetically Modified, Stress, Physiological.
Abstract
The diamine putrescine (Put) has been shown to accumulate in tree leaves in response to high Al and low Ca in the soil, leading to the suggestion that this response may provide a physiological advantage to leaf cells under conditions of Al stress. The increase in Put is reversed by Ca supplementation in the soil. Using two cell lines of poplar (Populus nigra x maximowiczii), one with constitutively high Put (resulting from transgenic expression of a mouse ornithine decarboxylase--called HP cells) and the other with low Put (control cells), we investigated the effects of reduced Ca (0.2-0.8 mM vs. 4 mM) and treatment with 0.1 mM Al on several biochemical parameters of cells. We found that in the presence of reduced Ca concentration, the HP cells were at a disadvantage as compared to control cells in that they showed greater reduction in mitochondrial activity and a reduction in the yield of cell mass. Upon addition of Al to the medium, the HP cells, however, showed a reversal of low-Ca effects. We conclude that due to increased ROS production in the HP cells, their tolerance to low Ca is compromised. Contrary to the expectation of deleterious effects, the HP cells showed an apparent advantage in the presence of Al in the medium, which could have come from reduced uptake of Al, enhanced extrusion of Al following its accumulation, and perhaps a reduction in Put catabolism as a result of a reduction in its biosynthesis.
DOI: 10.1016/j.plaphy.2010.04.010
PubMed: 20552726
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Minocha</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20552726</idno><idno type="pmid">20552726</idno><idno type="doi">10.1016/j.plaphy.2010.04.010</idno><idno type="wicri:Area/Main/Corpus">003156</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003156</idno><idno type="wicri:Area/Main/Curation">003156</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003156</idno><idno type="wicri:Area/Main/Exploration">003156</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The response of high and low polyamine-producing cell lines to aluminum and calcium stress.</title><author><name sortKey="Mohapatra, Sridev" sort="Mohapatra, Sridev" uniqKey="Mohapatra S" first="Sridev" last="Mohapatra">Sridev Mohapatra</name><affiliation wicri:level="2"><nlm:affiliation>Department of Biological Sciences, University of New Hampshire, Durham, NH 03824, USA.</nlm:affiliation><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Biological Sciences, University of New Hampshire, Durham, NH 03824</wicri:regionArea><placeName><region type="state">New Hampshire</region></placeName></affiliation></author><author><name sortKey="Cherry, Smita" sort="Cherry, Smita" uniqKey="Cherry S" first="Smita" last="Cherry">Smita Cherry</name></author><author><name sortKey="Minocha, Rakesh" sort="Minocha, Rakesh" uniqKey="Minocha R" first="Rakesh" last="Minocha">Rakesh Minocha</name></author><author><name sortKey="Majumdar, Rajtilak" sort="Majumdar, Rajtilak" uniqKey="Majumdar R" first="Rajtilak" last="Majumdar">Rajtilak Majumdar</name></author><author><name sortKey="Thangavel, Palaniswamy" sort="Thangavel, Palaniswamy" uniqKey="Thangavel P" first="Palaniswamy" last="Thangavel">Palaniswamy Thangavel</name></author><author><name sortKey="Long, Stephanie" sort="Long, Stephanie" uniqKey="Long S" first="Stephanie" last="Long">Stephanie Long</name></author><author><name sortKey="Minocha, Subhash C" sort="Minocha, Subhash C" uniqKey="Minocha S" first="Subhash C" last="Minocha">Subhash C. Minocha</name></author></analytic><series><title level="j">Plant physiology and biochemistry : PPB</title><idno type="eISSN">1873-2690</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Aluminum (metabolism)</term><term>Aluminum (pharmacology)</term><term>Animals (MeSH)</term><term>Calcium (metabolism)</term><term>Cell Line (MeSH)</term><term>Gene Expression (MeSH)</term><term>Mice (MeSH)</term><term>Mitochondria (drug effects)</term><term>Mitochondria (metabolism)</term><term>Ornithine Decarboxylase (metabolism)</term><term>Oxidative Stress (MeSH)</term><term>Plants, Genetically Modified (MeSH)</term><term>Populus (genetics)</term><term>Populus (metabolism)</term><term>Putrescine (metabolism)</term><term>Reactive Oxygen Species (metabolism)</term><term>Stress, Physiological (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Aluminium (métabolisme)</term><term>Aluminium (pharmacologie)</term><term>Animaux (MeSH)</term><term>Calcium (métabolisme)</term><term>Espèces réactives de l'oxygène (métabolisme)</term><term>Expression des gènes (MeSH)</term><term>Lignée cellulaire (MeSH)</term><term>Mitochondries (effets des médicaments et des substances chimiques)</term><term>Mitochondries (métabolisme)</term><term>Ornithine decarboxylase (métabolisme)</term><term>Populus (génétique)</term><term>Populus (métabolisme)</term><term>Putrescine (métabolisme)</term><term>Souris (MeSH)</term><term>Stress oxydatif (MeSH)</term><term>Stress physiologique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Aluminum</term><term>Calcium</term><term>Ornithine Decarboxylase</term><term>Putrescine</term><term>Reactive Oxygen Species</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Aluminum</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Mitochondria</term></keywords><keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Mitochondries</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Mitochondria</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Aluminium</term><term>Calcium</term><term>Espèces réactives de l'oxygène</term><term>Mitochondries</term><term>Ornithine decarboxylase</term><term>Populus</term><term>Putrescine</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Aluminium</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Cell Line</term><term>Gene Expression</term><term>Mice</term><term>Oxidative Stress</term><term>Plants, Genetically Modified</term><term>Stress, Physiological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Expression des gènes</term><term>Lignée cellulaire</term><term>Souris</term><term>Stress oxydatif</term><term>Stress physiologique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The diamine putrescine (Put) has been shown to accumulate in tree leaves in response to high Al and low Ca in the soil, leading to the suggestion that this response may provide a physiological advantage to leaf cells under conditions of Al stress. The increase in Put is reversed by Ca supplementation in the soil. Using two cell lines of poplar (Populus nigra x maximowiczii), one with constitutively high Put (resulting from transgenic expression of a mouse ornithine decarboxylase--called HP cells) and the other with low Put (control cells), we investigated the effects of reduced Ca (0.2-0.8 mM vs. 4 mM) and treatment with 0.1 mM Al on several biochemical parameters of cells. We found that in the presence of reduced Ca concentration, the HP cells were at a disadvantage as compared to control cells in that they showed greater reduction in mitochondrial activity and a reduction in the yield of cell mass. Upon addition of Al to the medium, the HP cells, however, showed a reversal of low-Ca effects. We conclude that due to increased ROS production in the HP cells, their tolerance to low Ca is compromised. Contrary to the expectation of deleterious effects, the HP cells showed an apparent advantage in the presence of Al in the medium, which could have come from reduced uptake of Al, enhanced extrusion of Al following its accumulation, and perhaps a reduction in Put catabolism as a result of a reduction in its biosynthesis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">20552726</PMID><DateCompleted><Year>2011</Year><Month>02</Month><Day>04</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Electronic">1873-2690</ISSN><JournalIssue CitedMedium="Internet"><Volume>48</Volume><Issue>7</Issue><PubDate><Year>2010</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Plant physiology and biochemistry : PPB</Title><ISOAbbreviation>Plant Physiol Biochem</ISOAbbreviation></Journal><ArticleTitle>The response of high and low polyamine-producing cell lines to aluminum and calcium stress.</ArticleTitle><Pagination><MedlinePgn>612-20</MedlinePgn></Pagination><Abstract><AbstractText>The diamine putrescine (Put) has been shown to accumulate in tree leaves in response to high Al and low Ca in the soil, leading to the suggestion that this response may provide a physiological advantage to leaf cells under conditions of Al stress. The increase in Put is reversed by Ca supplementation in the soil. Using two cell lines of poplar (Populus nigra x maximowiczii), one with constitutively high Put (resulting from transgenic expression of a mouse ornithine decarboxylase--called HP cells) and the other with low Put (control cells), we investigated the effects of reduced Ca (0.2-0.8 mM vs. 4 mM) and treatment with 0.1 mM Al on several biochemical parameters of cells. We found that in the presence of reduced Ca concentration, the HP cells were at a disadvantage as compared to control cells in that they showed greater reduction in mitochondrial activity and a reduction in the yield of cell mass. Upon addition of Al to the medium, the HP cells, however, showed a reversal of low-Ca effects. We conclude that due to increased ROS production in the HP cells, their tolerance to low Ca is compromised. Contrary to the expectation of deleterious effects, the HP cells showed an apparent advantage in the presence of Al in the medium, which could have come from reduced uptake of Al, enhanced extrusion of Al following its accumulation, and perhaps a reduction in Put catabolism as a result of a reduction in its biosynthesis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Mohapatra</LastName><ForeName>Sridev</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Department of Biological Sciences, University of New Hampshire, Durham, NH 03824, USA.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Cherry</LastName><ForeName>Smita</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Minocha</LastName><ForeName>Rakesh</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Majumdar</LastName><ForeName>Rajtilak</ForeName><Initials>R</Initials></Author><Author ValidYN="Y"><LastName>Thangavel</LastName><ForeName>Palaniswamy</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Long</LastName><ForeName>Stephanie</ForeName><Initials>S</Initials></Author><Author ValidYN="Y"><LastName>Minocha</LastName><ForeName>Subhash C</ForeName><Initials>SC</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>France</Country><MedlineTA>Plant Physiol Biochem</MedlineTA><NlmUniqueID>9882449</NlmUniqueID><ISSNLinking>0981-9428</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017382">Reactive Oxygen Species</NameOfSubstance></Chemical><Chemical><RegistryNumber>CPD4NFA903</RegistryNumber><NameOfSubstance UI="D000535">Aluminum</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 4.1.1.17</RegistryNumber><NameOfSubstance UI="D009955">Ornithine Decarboxylase</NameOfSubstance></Chemical><Chemical><RegistryNumber>SY7Q814VUP</RegistryNumber><NameOfSubstance UI="D002118">Calcium</NameOfSubstance></Chemical><Chemical><RegistryNumber>V10TVZ52E4</RegistryNumber><NameOfSubstance UI="D011700">Putrescine</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000535" MajorTopicYN="N">Aluminum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002118" MajorTopicYN="N">Calcium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D051379" MajorTopicYN="N">Mice</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008928" MajorTopicYN="N">Mitochondria</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009955" MajorTopicYN="N">Ornithine Decarboxylase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018384" MajorTopicYN="N">Oxidative Stress</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011700" MajorTopicYN="N">Putrescine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017382" MajorTopicYN="N">Reactive Oxygen Species</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013312" MajorTopicYN="Y">Stress, Physiological</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2010</Year><Month>6</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2010</Year><Month>6</Month><Day>17</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2011</Year><Month>2</Month><Day>5</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">20552726</ArticleId><ArticleId IdType="pii">S0981-9428(10)00110-5</ArticleId><ArticleId IdType="doi">10.1016/j.plaphy.2010.04.010</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>États-Unis</li></country><region><li>New Hampshire</li></region></list><tree><noCountry><name sortKey="Cherry, Smita" sort="Cherry, Smita" uniqKey="Cherry S" first="Smita" last="Cherry">Smita Cherry</name><name sortKey="Long, Stephanie" sort="Long, Stephanie" uniqKey="Long S" first="Stephanie" last="Long">Stephanie Long</name><name sortKey="Majumdar, Rajtilak" sort="Majumdar, Rajtilak" uniqKey="Majumdar R" first="Rajtilak" last="Majumdar">Rajtilak Majumdar</name><name sortKey="Minocha, Rakesh" sort="Minocha, Rakesh" uniqKey="Minocha R" first="Rakesh" last="Minocha">Rakesh Minocha</name><name sortKey="Minocha, Subhash C" sort="Minocha, Subhash C" uniqKey="Minocha S" first="Subhash C" last="Minocha">Subhash C. Minocha</name><name sortKey="Thangavel, Palaniswamy" sort="Thangavel, Palaniswamy" uniqKey="Thangavel P" first="Palaniswamy" last="Thangavel">Palaniswamy Thangavel</name></noCountry><country name="États-Unis"><region name="New Hampshire"><name sortKey="Mohapatra, Sridev" sort="Mohapatra, Sridev" uniqKey="Mohapatra S" first="Sridev" last="Mohapatra">Sridev Mohapatra</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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